Application
Detection of p19ARF in western blot of mouse embryonic fibroblast (MEF) lysate (25ug) using p19ARF Antibody [ (2ug/ml).Application
ICC/IF analysis of MEF cells using p19ARF antibody (green), alpha Tubulin (red) and DAPI (blue).Application
Western Blot: p19ARF Antibody - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.Application
antibody was tested on NIH 3T3 cells (which have a deletion of the p19ARF gene) as a negative control (-) and NIH 3T3 cells engineered to express HA-tagged p19ARF as a positive control (+). Total cell lysate (70ug/lane) was probed with 70 ng/mL of , which was detected using an anti-rabbit IgG-HRP conjugate.| Product Name | p19ARF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CDKN2AThe INK4a/ARF locus encodes two unrelated tumor suppressor proteins, p16INK4a and p19ARF that restrain cell growth by modifying the functions of the retinoblastoma protein and p53, respectively. It is among the most frequently mutated tumor suppressor loci in human cancer. Both p16INK4a and p19ARF act as cell proliferation inhibitors. The ARF gene, p19ARF in mouse and p14ARF in human, has become an important player in cell cycle regulation. In mice, tumor suppressor effects appear to be mediated by interactions between p19ARF and the p53 tumor-suppressor protein. p19ARF counters uncontrolled proliferation and oncogenic signals in p53-dependent pathways. Proteins encoded by the INK4a/ARF locus also play a role in Abelson virus transformation. Both p16INK4a and p19ARF are expressed in many cells as they emerge from the apoptotic crisis that characterizes the transformation process. Expression of p19ARF but not p16INK4a induces apoptosis in Ab-MLV-transformed pre-B cells. INK4a/ARF expression correlates with or precedes the emergence of cells expressing mutant p53. p19ARF is an important part in cellular defense against transforming signals from the Abl oncoprotein, providing direct evidence that the p19ARF-p53 regulatory loop plays an important role in lymphoma induction. |
| Immunogen | Synthetic peptide conjugated to KLH, within amino acids 54-75 of Mouse p19ARF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PIPWBChIP |
| For ELISA: Use at an assay dependent dilution. : |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Cyclin dependent kinase inhibitor 2A TP16 Cyclin dependent kinase 4 inhibitor A MTS1 Cell cycle negative regulator beta CMM2 CDKN2A MLM Cyclin dependent kinase inhibitor2A Cyclin dependent kinase inhibitor P12 Cyclin dependent kinase4 inhibitor |
|---|---|
| Target | CDKN2A |
| Tissue Specificity | On Western blot the antibody reveals IVT murine p19ARF and a band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs. |
Application
Detection of p19ARF in western blot of mouse embryonic fibroblast (MEF) lysate (25ug) using p19ARF Antibody [ (2ug/ml).
Application
ICC/IF analysis of MEF cells using p19ARF antibody (green), alpha Tubulin (red) and DAPI (blue).
Application
Western Blot: p19ARF Antibody - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.
Application
antibody was tested on NIH 3T3 cells (which have a deletion of the p19ARF gene) as a negative control (-) and NIH 3T3 cells engineered to express HA-tagged p19ARF as a positive control (+). Total cell lysate (70ug/lane) was probed with 70 ng/mL of , which was detected using an anti-rabbit IgG-HRP conjugate.| Positive Control | The blocking peptide for this antibody is available as GTX22659 |
|---|---|
| Application Notes | For ELISA: Use at an assay dependent dilution. : |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. Not recommended for use after expiration date - Five months. |
| Storage Buffer | Tris, pH 7.6 containing 0.1% sodium azide |
