Application
Immunohistochemical analysis of paraffin - embedded human lung adenocarcinoma showing nuclear location using P16 antibody with DAB staining.Application
Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.| Product Name | P16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A |
| Product description | p16 (cyclin-dependent kinase inhibitor 2A, INK4a) is a tumor suppressor protein. It is a specific inhibitor of Cdk 4 / Cdk 6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. Recent analyses of the p16 INK4a gene revealed homozygous deletions, nonsense, missense, or frameshift mutations in several human cancers. Although the frequency of p16 INK4a abnormalities is higher in tumor derived cell lines than in unselected primary tumors, significant subsets of clinical cases with aberrant p16 INK4a gene have been reported among melanomas, gliomas, esophageal, pancreatic, lung, and urinary bladder carcinomas, and some types of leukemia.1) Bai, F. et al. Mol. Cell. Biol.2003 23, 1269-1277. |
| Immunogen | Ni-NTA purified truncated recombinant P16 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CDKN2A |
|---|---|
| Alternative Names | ARF MLM P14 P16 P19 CMM2 INK4 MTS1 TP16 CDK4I CDKN2 INK4A MTS-1 P14ARF P19ARF P16INK4 P16INK4A P16-INK4A |
| Entrez Gene | 1029 |
|---|---|
| Protein Accession | NP_000068.1 |
Application
Immunohistochemical analysis of paraffin - embedded human lung adenocarcinoma showing nuclear location using P16 antibody with DAB staining.
Application
Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:200 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Human P16 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
