Application
Immunohistochemical analysis of paraffin - embedded human breast cancer (top), mouse brain tissue (middle) and mouse stomach tisue (bottom) showing nuclear location using P16 antibody with DAB staining.Application
Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.| Product Name | P16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF, MLM, P14, P16, P19, CMM2, INK4, MTS1, TP16, CDK4I, CDKN2, INK4A, MTS-1, P14ARF, P19ARF, P16INK4, P16INK4A, P16-INK4A |
| Product description | The progression of cells through the cell cycle is regulated by a family of protein kinases known as cyclin-dependent kinases (Cdks). The sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. The cyclins function as differentially expressed positive regulators of Cdks. Negative regulators of the cycle include the p53-inducible 21 kDa WAF1/Cip1 protein designated p21, Kip1 p27 and p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has recently been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines.1) Hunter, T. 1993. Cell 75: 839-841. |
| Immunogen | Ni-NTA purified truncated recombinant P16 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Symbol | CDKN2A |
|---|---|
| Alternative Names | ARF MLM P14 P16 P19 CMM2 INK4 MTS1 TP16 CDK4I CDKN2 INK4A MTS-1 P14ARF P19ARF P16INK4 P16INK4A P16-INK4A |
| Sequence Similarities | 2) Cat. No. 1415 - Mouse Stomach Tissue Lysate |
| Entrez Gene | 1029 |
|---|---|
| Protein Accession | NP_000068.1 |
Application
Immunohistochemical analysis of paraffin - embedded human breast cancer (top), mouse brain tissue (middle) and mouse stomach tisue (bottom) showing nuclear location using P16 antibody with DAB staining.
Application
Western blot analysis using P16 monoclonal antibody against truncated P16 recombinant protein.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | P16 (Mouse and Human) monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
