Application
Confocal microscopy of human HeLa cells using anti-p53 (BP53-12; FITC) . The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC MAb.| Product Name | p53 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [BP53-12] to p53This antibody is suitable in the research of p53 tumor suppressor gene function, in the detection of p53 expression and/or mutations in clinical specimens. The antibody is applicable as a possible marker in differential diagnosis and prognosis of p53 positive versus p53 negative human tumors. |
| Immunogen | Recombinant full length protein (wild type) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | BP53-12 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHCIHC-PIPWB |
| ELISA: Use at an assay dependant concentration. ICC: Use at an assay dependant concentration. IHC-P: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanPrimate |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Antigen NY CO 13 Cellular tumor antigen p53 Phosphoprotein p53 TP 53 TP53 Transformation Related Protein p53 TRP 53 TRP53 Tumor protein p53 Li Fraumeni syndrome Tumor suppressor p53 Tumour Protein p53 |
|---|---|
| Target | p53 |
| Tissue Specificity | This antibody reacts with the defined epitope (aa 16-25) on human p53, and recognizes p53/T complex. |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Confocal microscopy of human HeLa cells using anti-p53 (BP53-12; FITC) . The expression of p53 protein was enhanced by intercalating reagent. Cells were fixed and permeabilized before incubation with the p53-FITC MAb.| Application Notes | ELISA: Use at an assay dependant concentration. ICC: Use at an assay dependant concentration. IHC-P: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
