Application
Western blot analysis using Rabbit anti Paxillin [pY118] Polyclonal Antibody| Product Name | Paxillin (phospho Tyr118) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Paxillin ( phospho Y118 )Paxillin is a 68 kDa cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk). Tyrosine 118, a Crk binding site of paxillin, is phosphorylated during integrin-mediated cell adhesion. |
| Immunogen | Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 118. |
| Modification | p-Tyr118 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 68 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ16691 Paxillin (phospho Y118) Paxillin (phospho Tyr118) Paxillin (pY118) Paxillin (pTyr118) Paxillin phospho Y118 Paxillin phospho Tyr118 Phospho Paxillin (Y118) Phospho Paxillin (Tyr118) Phospho Paxillin (pY118) Phospho Paxillin (pTyr118) |
|---|---|
| Target | Paxillin |
| Cellular Localization | Cytoplasmic |
Application
Western blot analysis using Rabbit anti Paxillin [pY118] Polyclonal Antibody| Positive Control | NMµMG cells transfected with EGFP-tagged paxillin |
|---|---|
| Application Notes | WB: Use at a concentration of 0.5 - 1.0 µg/ml. Predicted molecular weight: 68 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.3 |
