Application
Immunofluorescent staining using Paxillin [pY31] phosphospecific antibody. Co-localization of paxillin [pY31] and actin in TGF-beta treated NMµMG cells. Green: actin stress fibers Orange: paxillin [pY31] Yellow: co-localization| Product Name | Paxillin (phospho Tyr31) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Paxillin ( phospho Y31 )Paxillin is a 68 kDa cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk 2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk). Tyrosine 31, a Crk binding site of paxillin, is phosphorylated during integrin-mediated cell adhesion. |
| Immunogen | Synthetic peptide (Human) derived from the region of human paxillin that contains tyrosine 31. |
| Modification | p-Tyr31 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: Use at a dilution of 1/1000. Predicted molecular weight: 68 kDa. Can be blocked with GTX25248 Paxillin phospho Y31 and non-phospho peptides (pair) Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ16691 Paxillin (phospho Y31) Paxillin (phospho Tyr31) Paxillin (pY31) Paxillin (pTyr31) Paxillin phospho Y31 Paxillin phospho Tyr31 Phospho Paxillin (Y31) Phospho Paxillin (Tyr31) Phospho Paxillin (pY31) Phospho Paxillin (pTyr31) |
|---|---|
| Target | Paxillin |
| Cellular Localization | Cytoplasmic |
Application
Immunofluorescent staining using Paxillin [pY31] phosphospecific antibody. Co-localization of paxillin [pY31] and actin in TGF-beta treated NMµMG cells. Green: actin stress fibers Orange: paxillin [pY31] Yellow: co-localization| Positive Control | NM_MG cells transfected with either the wild-type EGFP-tagged apxillin or a Y315F mutant |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000. Predicted molecular weight: 68 kDa. Can be blocked with GTX25248 Paxillin phospho Y31 and non-phospho peptides (pair) Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
