Application
Western blot analysis of Perforin using Jurkat cell lysate (Lane 1 & 2).| Product Name | Perforin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PerforinHuman perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~70 kDa Perforin in samples from human, mouse and rat origins. Reactivity to other species has not been determined.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLH2 HPLH2 PRF1 Cytolysin P1 MGC65093 PFP |
|---|---|
| Target | Perforin |
| Tissue Specificity | This antibody is specific for Perforin. |
Application
Western blot analysis of Perforin using Jurkat cell lysate (Lane 1 & 2).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~70 kDa Perforin in samples from human, mouse and rat origins. Reactivity to other species has not been determined.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20ºC. The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
