| Product Name | Perforin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [δG9] to PerforinHuman perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | δG9 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCIP |
| Immunoprecipitation (2-8 µg/ml), Flow cytometry (2-8 µg/ml), Immunohistochemistry (2-8 µg/ml for both frozen and paraffin sections), Immunocytochemistry (2-8 µg/ml),. However, the optimal conditions should be determined individually. Recognizes the 70 kDa perforin in human and primate.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLH2 HPLH2 PRF1 Cytolysin clone delta G9 P1 clone dG9 clone delta-G9 clone deltaG9 MGC65093 PFP |
|---|---|
| Target | Perforin |
| Tissue Specificity | This antibody is specific for Perforin. |
| Cellular Localization | Released from cytoplasmic granules of cytolytic T-lymphocytes. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. |
| Application Notes | Immunoprecipitation (2-8 µg/ml), Flow cytometry (2-8 µg/ml), Immunohistochemistry (2-8 µg/ml for both frozen and paraffin sections), Immunocytochemistry (2-8 µg/ml),. However, the optimal conditions should be determined individually. Recognizes the 70 kDa perforin in human and primate.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Stored at -20 °C for future use. |
| Storage Buffer | PBS (pH 7.2) containing 1 mg/ml BSA and 1.5 mM thimerosal and 50% glycerol. |
