Application
Immunofluorescence staining of methanol-fixed HeLa cells using Amyloid beta A4 (phospho-Thr743/668) Antibody.Application
Western blot analysis of extracts from cos-7 cells (Lane 2), using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The lane on the left is treated with antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Amyloid beta A4 (phospho-Thr743/668) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | Amyloid beta A4 (phospho Thr743/668) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A4, AD1, APP, APPI, Beta-amyloid protein 42 |
| Product description | Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity By similarity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1.1) Ming-Sum Lee J. Cell Biol., Oct 2003; 163: 83. |
| Immunogen | Amyloid beta A4 (phospho Thr743/668) antibody was raised against a peptide sequence around phosphorylation site of threonine 743 /668 (A-V-T (p)-P-E) derived from Human Amyloid beta A4. |
| Modification | p-Thr743/668 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APP |
|---|---|
| Alternative Names | A4 AD1 APP APPI Beta-amyloid protein 42 |
| Molecular Weight(MW) | 140 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Amyloid beta A4 only when phosphorylated at threonine 743/668. |
| Entrez Gene | 351 |
|---|---|
| Protein Accession | NP_000475.1 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Amyloid beta A4 (phospho-Thr743/668) Antibody.
Application
Western blot analysis of extracts from cos-7 cells (Lane 2), using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The lane on the left is treated with antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Amyloid beta A4 (phospho-Thr743/668) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
