Application
Western blot analysis of extracts from 293 cells treated with PMA using Phospho-C-RAF (Ser296) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | C-RAF (phospho Ser296) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C-Raf, CRAF, EC 2.7.11.1, RAF-1, kinase Raf1 |
| Product description | Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.1) Balan V, et al. (2006) Mol Biol Cell 17, 1141-53 |
| Immunogen | C-RAF (phospho Ser296) antibody was raised against a peptide sequence around phosphorylation site of serine 296 (S-S-S (p)-P-N) derived from Human C-RAF. |
| Modification | p-Ser296 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RAF1 |
|---|---|
| Alternative Names | C-Raf CRAF EC 2.7.11.1 RAF-1 kinase Raf1 |
| Molecular Weight(MW) | 74 kDa |
| Tissue Specificity | The antibody detects endogenous level of C-RAF only when phosphorylated at serine 296. |
| Entrez Gene | 5894 |
|---|---|
| Protein Accession | NP_002871.1 |
Application
Western blot analysis of extracts from 293 cells treated with PMA using Phospho-C-RAF (Ser296) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
