Application
Western blot analysis of lysed extracts from 293, HeLa and MDA cells using Caspase 3 Antibody.| Product Name | Caspase 3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CPP32, SCA-1, CPP32B, SCFR, Apopain |
| Product description | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.1) Gervais, F. G et al. Cell 97: 395-406, 1999. Nicholson, D. W. et al. (1995) Nature 376, 37-43. |
| Immunogen | Caspase 3 antibody was raised against a peptide sequence around aa.29~33 (S-G-I-S-L) derived from Caspase 3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP3 |
|---|---|
| Alternative Names | CPP32 SCA-1 CPP32B SCFR Apopain |
| Molecular Weight(MW) | 35 19 17 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Caspase 3 protein. |
| Entrez Gene | 836 |
|---|---|
| Protein Accession | NP_004337.2 |
Application
Western blot analysis of lysed extracts from 293, HeLa and MDA cells using Caspase 3 Antibody.| Application Notes | Western Blot: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
