Application
Western Blot analysis of Caspase-3 inmouse RAW cells using Caspase-3 monoclonal Antibody.Application
Immunohistochemistry staining of Caspase-3 in thymus tissue using Caspase-3 Monoclonal Antibody.| Product Name | Caspase-3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32- kDa protein (2-4). Caspase-3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc.. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17- kDa and a small subunit of 12- kDa. Caspase 3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase 3 proteolytically cleaves and activates other proteins. Caspase 3 has been associated with apoptosis. |
| Immunogen | Full length recombinant human Caspase-3 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| Caspase-3 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CASP3 |
|---|---|
| Alternative Names | CASP3 Apopain CASP-3 Caspase-3 CPP32B CPP32 Cysteine protease CPP32 SCA-1 SREBP cleavage activity 1 Yama Procaspase3 Caspase 3 CPP-32 PARP cleavage protease Protein Yama |
Application
Western Blot analysis of Caspase-3 inmouse RAW cells using Caspase-3 monoclonal Antibody.
Application
Immunohistochemistry staining of Caspase-3 in thymus tissue using Caspase-3 Monoclonal Antibody.| Application Notes | Caspase-3 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store Caspase-3 antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.05% BSA, 0.05% sodium azide. |
