Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Caspase 9 (phospho Thr125) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APAF-3, MCH6, RNCASP9, CASP-9, ICE-LAP6 |
| Product description | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.1) Duan H., J. Biol. Chem. 271:16720-16724(1996). |
| Immunogen | Caspase 9 (phospho Thr125) antibody was raised against a peptide sequence around phosphorylation site of threonine 125 (P-E-T (p)-P-R) derived from Human Caspase 9. |
| Modification | p-Thr125 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP9 |
|---|---|
| Alternative Names | APAF-3 MCH6 RNCASP9 CASP-9 ICE-LAP6 |
| Molecular Weight(MW) | 47 kDa |
| Tissue Specificity | The antiserum was produced against synthesized phosphopeptide derived from human Caspase 9 around the phosphorylation site of threonine 125 (P-E-TP-P-R). |
| Entrez Gene | 842 |
|---|---|
| Protein Accession | NP_001220.2 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Caspase 9 (Phospho-Thr125) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HeLa cells treated with TNF using Caspase 9 (Phospho-Thr125) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
