Application
Western blot analysis of lysed extracts from 293 and HeLa cells using Caspase 9 Antibody.| Product Name | Caspase 9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1, ICE-like apoptotic protease 6 |
| Product description | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.1) Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223. Li, P. et al. (1997) Cell 91, 479-489. |
| Immunogen | Caspase 9 antibody was raised against a peptide sequence around aa.301~305 (T-S-P-E-D) derived from Caspase 9. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP9 |
|---|---|
| Alternative Names | MCH6 APAF3 APAF-3 PPP1R56 ICE-LAP6 DXS423E KIAA0178 SB1.8 SMC1 SMC1L1 ICE-like apoptotic protease 6 |
| Molecular Weight(MW) | 47, 37, 35 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Caspase 9 protein. |
| Entrez Gene | 842 |
|---|---|
| Protein Accession | NP_001220.2 |
Application
Western blot analysis of lysed extracts from 293 and HeLa cells using Caspase 9 Antibody.| Application Notes | Western Blot: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
