Application
Immunocytochemistry staining of K562 cells using Caspase-9 antibody at 2 μg/mL.Application
Immunocytochemistry of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 μg/ml.Application
Immunofluorescence of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 μg/ml.Application
Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 μg/mL.Application
Western blot analysis of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody at 1 μg/mL.| Product Name | Caspase-9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9 |
| Product description | Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.1) Duan H, Orth K, Chinnaiyan AM, et al. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 1996; 271:16720-4 |
| Immunogen | Caspase-9 antibody was raised against a 20 amino acid peptide near the center of human Caspase-9. The immunogen is located within amino acids 290 - 340 of Caspase-9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIPWB |
| Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP9 |
|---|---|
| Alternative Names | Caspase-9 Antibody: MCH6 APAF3 APAF-3 PPP1R56 ICE-LAP6 MCH6 Caspase-9 Apoptotic protease Mch-6 CASP-9 |
| Molecular Weight(MW) | Predicted: 46 kDa Observed: 45 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family. |
Application
Immunocytochemistry staining of K562 cells using Caspase-9 antibody at 2 μg/mL.
Application
Immunocytochemistry of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 μg/ml.
Application
Immunofluorescence of Caspase-9 in HeLa cells with Caspase-9 antibody at 5 μg/ml.
Application
Immunofluorescence of Caspase-9 in K562 cells with Caspase-9 antibody at 20 μg/mL.
Application
Western blot analysis of caspase-9 in HeLa whole cell lysate with Caspase-9 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide. |
