Application
Immunohistochemistry staining of Caspase-9 in heart tissue using Caspase-9 Antibody.| Product Name | Caspase-9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CASP9, APAF3, CASPASE-9c, ICE-LAP6, MCH6, ICE-like apoptotic protease 6, APAF-3, Apoptotic protease Mch-6, CASP-9, Caspase 9, Caspase-9, PPP1R56 |
| Product description | Caspase-9 and Apaf-1 bind to each other, which leads to Caspase-9 activation. Activated caspase-9 cleaves and activates Caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Recently, a novel isoform of rat Caspase-9 has been identified in which the C terminus of full-length Caspase-9 is replaced with an alternative peptide sequence. This protein called, Casp-9-CTD (where CTD is carboxyl-terminal divergent) is expressed in multiple tissues, with the relative highest statement observed in ovary and heart. The variant C terminus of Casp-9-CTD in rat is derived from an alternative exon of the rat Caspase-9 gene. Casp-9-CTD was found primarily in the cytoplasm and was not detected in the nucleus. Caspase-9-CTD proenzyme is not processed in the cells and lacks apoptotic activity. The Casp-9-CTD transfected cells are resistant to caspases induction by cytochrome c, suggesting that Casp-9-CTD acts as a dominant-negative variant. Caspase 9 is a cysteine C14 endopeptidase that mediates oncogene and drug-induced apoptosis. It is activated upon binding to Apaf-1/cytochrome c and inhibited upon phosphorylation by the serine/ threonine kinase Akt. Activated caspase-9 cleaves downstream caspases such as Caspase-3, -6 and -7 initiating the caspase cascade. Like other caspases, Caspase-9 plays a critical role in mediating apoptosis during normal brain development. Caspase-9 also cleaves amyloid beta-protein precursors, principal constituent of senile plaques, and is involved in neuronal death observed in Alzheimer disease. |
| Immunogen | Caspase-9 antibody was raised against amino acids 345 - 359 of Caspase-9 (Rat). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Caspase-9 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | CASP9 |
|---|---|
| Alternative Names | CASP9 APAF3 CASPASE-9c ICE-LAP6 MCH6 ICE-like apoptotic protease 6 APAF-3 Apoptotic protease Mch-6 CASP-9 Caspase 9 Caspase-9 PPP1R56 |
| Tissue Specificity | A 45 kD band is detected. |
Application
Immunohistochemistry staining of Caspase-9 in heart tissue using Caspase-9 Antibody.| Application Notes | Caspase-9 antibody can be used in ELISA, Western Blot starting at 1:500 - 1:1000, and immunohistochemistry starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-9 antibody can be stored short term 4 ˚C. For long term storage aliquot and store at -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | PBS, 0.02% sodium azide. |
