Application
Western blot analysis of caspase-9 in HeLa whole cell lysate with caspase-9 antibody at 1:1000 dilution.| Product Name | Caspase-9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9 |
| Product description | Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.1) Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4 |
| Immunogen | Caspase-9 antibody was raised against a 16 amino acid peptide near the amino terminus of human Caspase-9. The immunogen is located within amino acids 30 - 80 of Caspase-9. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| WB:1:500~1:2000 IP:1:100~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP9 |
|---|---|
| Alternative Names | Caspase-9 Antibody: MCH6 APAF3 APAF-3 PPP1R56 ICE-LAP6 MCH6 Caspase-9 Apoptotic protease Mch-6 CASP-9 |
| Molecular Weight(MW) | 46 kDa |
| Tissue Specificity | It has no cross response to other members in caspase family. |
Application
Western blot analysis of caspase-9 in HeLa whole cell lysate with caspase-9 antibody at 1:1000 dilution.| Positive Control | HeLa Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide. |
