Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Product Name | EGLN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Egl nine homolog 1, Hypoxia-inducible factor prolyl hydroxylase 2, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, Prolyl hydroxylase domain-containing protein 2, PHD2, SM-20, EGLN1 ( HGNC:1232 ), C1orf12 |
| Product description | The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3).1) Aggarwal, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(44):18961-18966(2010) |
| Immunogen | This EGLN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-425 amino acids from the C-terminal region of human EGLN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EGLN1 |
|---|---|
| Alternative Names | Egl nine homolog 1 Hypoxia-inducible factor prolyl hydroxylase 2 HIF-PH2 HIF-prolyl hydroxylase 2 HPH-2 Prolyl hydroxylase domain-containing protein 2 PHD2 SM-20 EGLN1 ( HGNC:1232 ) C1orf12 |
| Molecular Weight(MW) | 46 kDa |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
