Application
Western Blot at 1:2000 dilution Lane 1: mouse skeletal muscle lysates Lane 2: rat skeletal muscle lysates Lane 3: Hela whole cell lysates Lane 4: HepG2 whole cell lysates Lane 5: SH-SY5Y whole cell lysates Lysates/proteins at 20 ug per lane.Application
Antibody staining LGR5 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Antibody staining LGR5 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Antibody staining LGR5 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).Application
Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IApplication
Overlay histogram showing SH-SY5Y cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-RabbitApplication
Overlay histogram showing SH-SY5Y cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit| Product Name | LGR5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67 |
| Product description | LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues. Stem cell marker of the intestinal epithelium and the hair follicle. Target gene of Wnt signaling. Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.1) References for protein: |
| Immunogen | This LGR5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 689-719 amino acids from human LGR5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| WB:1:500~1:2000 IF:1:100~1:500 IHC:1:50~1:100 FACS:1:200~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LGR5 |
|---|---|
| Alternative Names | Leucine-rich repeat-containing G-protein coupled receptor 5 G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 LGR5 GPR49 GPR67 |
| Molecular Weight(MW) | 100 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: mouse skeletal muscle lysates Lane 2: rat skeletal muscle lysates Lane 3: Hela whole cell lysates Lane 4: HepG2 whole cell lysates Lane 5: SH-SY5Y whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Antibody staining LGR5 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Antibody staining LGR5 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Antibody staining LGR5 in human brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Application
Overlay histogram showing HepG2 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I
Application
Overlay histogram showing SH-SY5Y cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit
Application
Overlay histogram showing SH-SY5Y cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit| Application Notes | WB:1:500~1:2000 IF:1:100~1:500 IHC:1:50~1:100 FACS:1:200~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
