WB
Western blot analysis of LGR5 using anti-LGR5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LGR5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of LGR5 using anti-LGR5 antibody. LGR5 was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-LGR5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of LGR5 using anti-LGR5 antibody. LGR5 was detected in a paraffin-embedded section of rat small intestine tissue. The tissue section was incubated with rabbit anti-LGR5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of LGR5 using anti-LGR5 antibody. LGR5 was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-LGR5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of 293T cells using anti-LGR5 antibody. Overlay histogram showing 293T cells stained with Rabbit polyclonal antibody to LGR5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LGR5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to LGR5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human LGR5 recombinant protein (Position: R26-L907). Human LGR5 shares 87% and 87.1% amino acid (aa) sequence identity with mouse and rat LGR5, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | LGR5 |
|---|---|
| Gene Synonyms | FEX HG38 GPR49 GPR67 GRP49 |
| Gene Full Name | leucine rich repeat containing G protein-coupled receptor 5 |
| Gene Summary | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | LGR5 GPR49 GPR67 Leucine-rich repeat-containing G-protein coupled receptor 5 G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 |
| Molecular Weight(MW) | 100,97,92 kDa(Observed band size:130 kDa) |
| Cellular Localization | Cell membrane,Golgi apparatus,Membrane. |
WB
Western blot analysis of LGR5 using anti-LGR5 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-LGR5 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody . The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of LGR5 using anti-LGR5 antibody. LGR5 was detected in a paraffin-embedded section of mouse small intestine tissue. The tissue section was incubated with rabbit anti-LGR5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of LGR5 using anti-LGR5 antibody. LGR5 was detected in a paraffin-embedded section of rat small intestine tissue. The tissue section was incubated with rabbit anti-LGR5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of LGR5 using anti-LGR5 antibody. LGR5 was detected in a paraffin-embedded section of human kidney tissue. The tissue section was incubated with rabbit anti-LGR5 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of 293T cells using anti-LGR5 antibody. Overlay histogram showing 293T cells stained with Rabbit polyclonal antibody to LGR5 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LGR5 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
