WB
Western blot analysis of extracts (15ug) from 2 Human tissue by using anti-LGR5 monoclonal antibody (left lane: Brain right lane: Testis) (1:200).IHC
Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-LGR5 at 1:400 with GBI Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0 using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.ICC/IF
Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY mouse LGR5(1:100).FC
Flow cytometric analysis of stable expression LGR5 HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-LGR5 antibody (1:100) surface stained on live cells.| Product Name | LGR5 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:100-1:150 ICC/IF:1:100 FC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | LGR5 |
|---|---|
| Gene Synonyms | FEX HG38 GPR49 GPR67 GRP49 |
| Gene Full Name | leucine rich repeat containing G protein-coupled receptor 5 |
| Gene Summary | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | FEX GPR49 GPR67 GRP49 HG38 |
| Molecular Weight(MW) | 99.8 kDa |
WB
Western blot analysis of extracts (15ug) from 2 Human tissue by using anti-LGR5 monoclonal antibody (left lane: Brain right lane: Testis) (1:200).
IHC
Immunohistochemical staining of paraffin-embedded human colon cancer using mouse anti-LGR5 at 1:400 with GBI Polink2 Broad HRP DAB detection kit; pretreatment of tissue prior to stain with heat-induced epitope retrieval buffer from GBI Labs TEE pH 9.0 using pressure chamber for 3 minutes at 110C is required for optimal staining. Shown here strong cytoplamic tumor cells.
ICC/IF
Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of MCF7 cells transiently transfected by pCMV6-ENTRY mouse LGR5(1:100).
FC
Flow cytometric analysis of stable expression LGR5 HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-LGR5 antibody (1:100) surface stained on live cells.| Application Notes | WB:1:200-1:2000 IHC:1:100-1:150 ICC/IF:1:100 FC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
