WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LGR5 GPR49 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human small intestine was stained with anti-LGR5 GPR49 Rabbit antibody.| Product Name | LGR5/GPR49 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | LGR5 |
|---|---|
| Gene Synonyms | FEX HG38 GPR49 GPR67 GRP49 |
| Gene Full Name | leucine rich repeat containing G protein-coupled receptor 5 |
| Gene Summary | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | LGR5 GPR49 GPR67 |
| Molecular Weight(MW) | 100 kDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Golgi apparatus , trans-Golgi network membrane; Multi-pass membrane protein. Rapidly and constitutively internalized to the trans-Golgi network at steady state. Internalization to the trans-Golgi network may be the result of phosphorylation at Ser-861 and Ser-864; however , the phosphorylation event has not been proven (PubMed:23439653) . . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-LGR5 GPR49 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human small intestine was stained with anti-LGR5 GPR49 Rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
