WB
Western blot analysis of extracts (10ug) from 7 different transiently transfected HEK293T cell lysates by using anti-LGR5 monoclonal antibody.IHC
Immunohistochemical staining of paraffin embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min,(1:150).ICC/IF
Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of MCF7 cells transiently transfected by pCMV6 ENTRY human LGR5 (1:100).FC
Flow cytometric analysis of stable expression LGR5 HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-LGR5 antibody (1:100) surface stained on live cells.| Product Name | LGR5 Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:1000~1:2000 IHC:1:100~1:150 ICC/IF:1:100 FC:1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A/G |
| Gene Symbol | LGR5 |
|---|---|
| Gene Synonyms | FEX HG38 GPR49 GPR67 GRP49 |
| Gene Full Name | leucine rich repeat containing G protein-coupled receptor 5 |
| Gene Summary | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| Alternative Names | FEX GPR49 GPR67 GRP49 HG38 |
| Molecular Weight(MW) | 99.8 kDa |
| Cellular Localization | Cell membrane,Golgi apparatus,Membrane. |
| SwissProt ID | O75473 |
|---|
WB
Western blot analysis of extracts (10ug) from 7 different transiently transfected HEK293T cell lysates by using anti-LGR5 monoclonal antibody.
IHC
Immunohistochemical staining of paraffin embedded Adenocarcinoma of Human colon tissue using anti-LGR5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min,(1:150).
ICC/IF
Anti-LGR5 mouse monoclonal antibody immunofluorescent staining of MCF7 cells transiently transfected by pCMV6 ENTRY human LGR5 (1:100).
FC
Flow cytometric analysis of stable expression LGR5 HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-LGR5 antibody (1:100) surface stained on live cells.| Application Notes | WB:1:1000~1:2000 IHC:1:100~1:150 ICC/IF:1:100 FC:1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
