Application
Confocal immunofluorescent analysis of LGR5/GPR49 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).| Product Name | LGR5/GPR49 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67 |
| Product description | LGR5/GPR49 is an orphan receptor. It may be an important receptor for signals controlling growth and differentiation of specific embryonic tissues.1) Barker,N., Nature 449 (7165), 1003-1007 (2007) |
| Immunogen | This LGR5/GPR49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-270 amino acids from the N-terminal region of human LGR5/GPR49. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | LGR5 |
|---|---|
| Alternative Names | Leucine-rich repeat-containing G-protein coupled receptor 5 G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 LGR5 GPR49 GPR67 |
| Molecular Weight(MW) | 100 kDa |
Application
Confocal immunofluorescent analysis of LGR5/GPR49 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of HepG2 cells using LGR5/GPR49 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5/GPR49 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow)(Kindly offered by Dr. Li).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
