Application
Antibody used in IHC on Human stomach at 1:500.Application
Antibody used in IHC on Pig stomach at 1:500.Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.| Product Name | MUC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | MUC1 is a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MUC1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| MUC1 antibody can be used for detection of MUC1 by ELISA at 1:312500. MUC1 antibody can be used for detection of MUC1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MUC1 |
|---|---|
| Alternative Names | MUC1 CD227 EMA PEM PUM mucin MCD KL-6 MAM6 MCKD PEMT H23AG MCKD1 MUC-1 ADMCKD ADMCKD1 CA 15-3 MUC-1/X MUC1/ZD MUC-1/SEC |
| Molecular Weight(MW) | 22 kDa, 28 kDa, 28 kDa, 16 kDa, 17 kDa, 30 kDa |
| Entrez Gene | 4582 |
|---|---|
| Protein Accession | NP_001037855 |
Application
Antibody used in IHC on Human stomach at 1:500.
Application
Antibody used in IHC on Pig stomach at 1:500.
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.
Application
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | MUC1 antibody can be used for detection of MUC1 by ELISA at 1:312500. MUC1 antibody can be used for detection of MUC1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store MUC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
