Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.Application
Antibody used in WB on Human MCF7 cells at 1 ug/ml.Application
Antibody used in IHC on Human Tonsil at 5.0 ug/ml.| Product Name | NCOA3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NCOA3 is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.The protein encoded by this gene is a nuclear receptor coactivator that interacts with nuclear hormone receptors to enhance their transcriptional activator functions. The encoded protein has histone acetyltransferase activity and recruits p300/CBP-associated factor and CREB binding protein as part of a multisubunit coactivation complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. Two transcript variants encoding different isoforms have been found for this gene. In addition, a polymorphic repeat region is found in the C-terminus of the encoded protein.1) Luo, J.H., (2008) Int. J. Cancer 122 (11), 2554-2561. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCOA3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NCOA3 antibody can be used for detection of NCOA3 by ELISA at 1:312500. NCOA3 antibody can be used for detection of NCOA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCOA3 |
|---|---|
| Alternative Names | NCOA3 ACTR AIB-1 AIB1 CAGH16 CTG26 KAT13B MGC141848 RAC3 SRC-1 SRC3 TNRC14 TNRC16 TRAM-1 pCIP SRC-3 bHLHe42 |
| Molecular Weight(MW) | 155 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Application
Antibody used in WB on Human MCF7 cells at 1 ug/ml.
Application
Antibody used in IHC on Human Tonsil at 5.0 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | NCOA3 antibody can be used for detection of NCOA3 by ELISA at 1:312500. NCOA3 antibody can be used for detection of NCOA3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NCOA3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
