Application
Western blot analysis of extract from HeLa cells using REST Antibody.| Product Name | REST Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | XBR, NRSF, RNF53, XBR, REST |
| Product description | Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier.1) Chong J. A. et. al. (1995) Cell 80:949-957Schoenherr C. J. et. al. (1995) Science 267:1360-1363Scholl T. et. al. (1996) J. Immunol. 156:1448-1457Lunyak V. V. et. al. (2002) Science 298:1747-1752 |
| Immunogen | REST antibody was raised against a peptide sequence around aa.873~877 (R-E-E-A-S) derived from Human REST. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | REST |
|---|---|
| Alternative Names | XBR NRSF RNF53 XBR REST |
| Molecular Weight(MW) | 121 kDa |
| Tissue Specificity | This antibody detects endogenous level of total REST protein. |
| Entrez Gene | 5978 |
|---|---|
| Protein Accession | NP_001180437.1 |
Application
Western blot analysis of extract from HeLa cells using REST Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
