WB
Various lysates were subjected to SDS PAGE followed by western blot with REST antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using REST antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) , CL594-Phalloidin (red).CHIP
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of REST or 5 ug of Normal Rabbit IgG, and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.| Product Name | REST Recombinant monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human REST protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFWB |
| WB:1:2000-1:10000 ICC/IF:1:200-1:800 CHIP:1:10-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | REST |
|---|---|
| Gene Synonyms | WT6 XBR HGF5 NRSF DFNA27 GINGF5 |
| Gene Full Name | RE1 silencing transcription factor |
| Gene Summary | This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018] |
| Alternative Names | 241789D3 NRSF X2 box repressor XBR |
| Molecular Weight(MW) | 1097 aa, 122 kDa (Observed band size: 200 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Various lysates were subjected to SDS PAGE followed by western blot with REST antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using REST antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) , CL594-Phalloidin (red).
CHIP
Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of REST or 5 ug of Normal Rabbit IgG, and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.| Application Notes | WB:1:2000-1:10000 ICC/IF:1:200-1:800 CHIP:1:10-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
