Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Raji whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | REST Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor, REST, NRSF, XBR |
| Product description | Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. |
| Immunogen | This REST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 542-570 amino acids from the Central region of human REST. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | REST |
|---|---|
| Alternative Names | RE1-silencing transcription factor Neural-restrictive silencer factor X2 box repressor REST NRSF XBR |
| Molecular Weight(MW) | 122 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: Raji whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
