WB
WB result of REST antibody (1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REST transfected HEK-293 cells.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using REST antibody at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using REST antibody at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IP
IP result of anti-REST (IP:4ug; Detection:1:15000) with HeLa cells lysate 1750 ug.| Product Name | REST Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human REST protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:500-1:3000 IP :0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC :1:400-1:1600 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | REST |
|---|---|
| Gene Synonyms | WT6 XBR HGF5 NRSF DFNA27 GINGF5 |
| Gene Full Name | RE1 silencing transcription factor |
| Gene Summary | This gene was initially identified as a transcriptional repressor that represses neuronal genes in non-neuronal tissues. However, depending on the cellular context, this gene can act as either an oncogene or a tumor suppressor. The encoded protein is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regulator of neurogenesis. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2018] |
| Alternative Names | NRSF X2 box repressor XBR |
| Molecular Weight(MW) | 1097 aa, 122 kDa (Observed band size: 200 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
WB result of REST antibody (1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REST transfected HEK-293 cells.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using REST antibody at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using REST antibody at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP
IP result of anti-REST (IP:4ug; Detection:1:15000) with HeLa cells lysate 1750 ug.| Application Notes | WB:1:500-1:3000 IP :0.5-4.0 ug for 1.0-3.0 mg of total protein lysate IHC :1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
