Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Product Name | SCYE1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SCYE1, p43, HLD3, EMAP2, SCYE1, EMAPII |
| Product description | SCYE1 is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex. Therefore, pro-SCYE1 may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.The protein encoded by this gene is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor of SCYE1 (pro-SCYE1) is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex. Therefore, pro-SCYE1 may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.1) Tandle, A.T., (2005) Cytokine 30 (6), 347-358. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SCYE1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| SCYE1 antibody can be used for detection of SCYE1 by ELISA at 1:62500. SCYE1 antibody can be used for detection of SCYE1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AIMP1 |
|---|---|
| Alternative Names | SCYE1 p43 HLD3 EMAP2 SCYE1 EMAPII |
| Molecular Weight(MW) | 34 kDa |
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | SCYE1 antibody can be used for detection of SCYE1 by ELISA at 1:62500. SCYE1 antibody can be used for detection of SCYE1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SCYE1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
