WB
Western blotting analysis using AIMP1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AIMP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.ICC/IF
Immunocytochemical staining of HT-1080 cells with AIMP1 antibody. Nuclei were stained blue with DAPI; AIMP1was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.FC
Flow cytometric analysis of AIMP1 expression in HT-1080 cells using AIMP1 antibody. Green, isotype control; red, AIMP1.| Product Name | AIMP1 Recombinant Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from Human AIMP1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:1000-1:5000 FC:1:200-1:2000 ICC/IF:1:100-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AIMP1 |
|---|---|
| Gene Synonyms | p43 HLD3 EMAP2 SCYE1 EMAPII |
| Gene Full Name | aminoacyl tRNA synthetase complex interacting multifunctional protein 1 |
| Gene Summary | The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008] |
| Alternative Names | AIMP1 Aminoacyl TRNA Synthetase Complex Interacting Multifunctional Protein 1 EMAPII SCYE1 P43 Small Inducible Cytokine Subfamily E Member 1 (Endothelial Monocyte-Activating) Aminoacyl TRNA Synthase Complex-Interacting Multifunctional Protein 1 Multisynthetase Complex Auxiliary Component P43 Multisynthase Complex Auxiliary Component P43 ARS-Interacting Multifunctional Protein 1 EMAP-2 EMAP2 Endothelial-Monocyte Activating Polypeptide II Endothelial Monocyte-Activating Polypeptide EMAP II HLD3 |
| Cellular Localization | Nucleus. Cytoplasm, cytosol. Secreted. Endoplasmic reticulum. Golgi apparatus. |
WB
Western blotting analysis using AIMP1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AIMP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
ICC/IF
Immunocytochemical staining of HT-1080 cells with AIMP1 antibody. Nuclei were stained blue with DAPI; AIMP1was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
FC
Flow cytometric analysis of AIMP1 expression in HT-1080 cells using AIMP1 antibody. Green, isotype control; red, AIMP1.| Application Notes | WB:1:1000-1:5000 FC:1:200-1:2000 ICC/IF:1:100-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
