WB
Western Blot: Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate. All lanes: AIMP1 antibody at 3.7µg/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.IHC
IHC image of AIMP1 Antibody diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.IHC
IHC image of AIMP1 Antibody diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.ICC/IF
Immunofluorescence staining of Hela cells with AIMP1 Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-AIMP1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 protein (25-180AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:5000 IHC:1:200-1:500 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | AIMP1 |
|---|---|
| Gene Synonyms | p43 HLD3 EMAP2 SCYE1 EMAPII |
| Gene Full Name | aminoacyl tRNA synthetase complex interacting multifunctional protein 1 |
| Gene Summary | The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20. [provided by RefSeq, Dec 2008] |
| Alternative Names | AIMP 1 antibody Aimp1 antibody AIMP1/p43 antibody AIMP1_HUMAN antibody Aminoacyl tRNA synthetase complex-interacting multifunctional protein 1 antibody ARS interacting multifunctional protein 1 antibody EMAP 2 antibody EMAP-2 antibody EMAP-II antibody EMAP2 antibody EMAPII antibody Endothelial monocyte activating polypeptide 2 antibody Endothelial monocyte activating polypeptide antibody Endothelial monocyte-activating polypeptide 2 antibody Endothelial monocyte-activating polypeptide II antibody HLD3 antibody Multisynthase complex auxiliary component p43 antibody Multisynthetase complex auxiliary component p43 antibody OTTHUMP00000219673 antibody p43 antibody Scye 1 antibody Scye1 antibody Scye1 formerly antibody Small inducible cytokine subfamily E member 1 antibody Small inducible cytokine subfamily E member 1 (endothelial monocyte-activating) antibody Small inducible cytokine subfamily E member 1 formerly antibody |
| Molecular Weight(MW) | 35,38 kDa(Observed band size: 35 kDa) |
| Cellular Localization | Nucleus. Cytoplasm, cytosol. Secreted. Endoplasmic reticulum. Golgi apparatus. |
WB
Western Blot: Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate. All lanes: AIMP1 antibody at 3.7µg/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
IHC image of AIMP1 Antibody diluted at 1:200 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
IHC image of AIMP1 Antibody diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ICC/IF
Immunofluorescence staining of Hela cells with AIMP1 Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:5000 IHC:1:200-1:500 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
