Application
Western blot analysis of SFRS2 Antibody in mouse stomach tissue lysates (35ug/lane)| Product Name | SFRS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.1) Merdzhanova,G., Cell Death Differ. 15 (12), 1815-1823 (2008) |
| Immunogen | This SFRS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the Central region of human SFRS2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | SRSF2 |
|---|---|
| Alternative Names | Serine/arginine-rich splicing factor 2 Protein PR264 Splicing component 35 kDa Splicing factor SC35 SC-35 Splicing factor arginine/serine-rich 2 SRSF2 SFRS2 |
| Molecular Weight(MW) | 25 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of SFRS2 Antibody in mouse stomach tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
