WB
Western blot analysis of SR expression in HL-60(A),K-562(B),HeLa(C),PC-3(D) andMOLT-4(E)nuclear extracts.IHC
Immunoperoxidase staining of formalin fixed and paraffin embedded external genital/anal skin tissue showed staining of epidermal cell nuclei.ICC/IF
Immunofluorescence staining of HeLa cells fixed with methanol showed nuclear localization.| Product Name | Mouse Monoclonal antibody to SR |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against full length SR of Xenopus origin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanXenopus |
| Concentration | 0.1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SRSF2 |
|---|---|
| Gene Synonyms | SC35 PR264 SC-35 SFRS2 SFRS2A SRp30b |
| Gene Full Name | serine and arginine rich splicing factor 2 |
| Gene Summary | The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010] |
| Molecular Weight(MW) | 34 kDa |
| Cellular Localization | Nuclear |
WB
Western blot analysis of SR expression in HL-60(A),K-562(B),HeLa(C),PC-3(D) andMOLT-4(E)nuclear extracts.
IHC
Immunoperoxidase staining of formalin fixed and paraffin embedded external genital/anal skin tissue showed staining of epidermal cell nuclei.
ICC/IF
Immunofluorescence staining of HeLa cells fixed with methanol showed nuclear localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
