WB
Various lysates were subjected to SDS PAGE followed by western blot with SFRS2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin embedded human lung cancer tissue slide using SFRS2 antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS2 antibody at dilution of 1:3000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L), CL594-phalloidin (red).IP
IP result of anti-SFRS2 (IP:4ug; Detection:1:300) with mouse brain tissue lysate 4000ug.| Product Name | SFRS2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:8000 IHC:1:50-1:500 ICC:1:1500-1:6000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.6mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SRSF2 |
|---|---|
| Gene Synonyms | SC35 PR264 SC-35 SFRS2 SFRS2A SRp30b |
| Gene Full Name | serine and arginine rich splicing factor 2 |
| Gene Summary | The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two transcript variants encoding the same protein and one non-coding transcript variant have been found for this gene. In addition, a pseudogene of this gene has been found on chromosome 11. [provided by RefSeq, Sep 2010] |
| Alternative Names | PR264 Protein PR264 SC 35 SC35 SC-35 |
| Molecular Weight(MW) | 25kDa(Observed Molecular Weight 25-35 kDa) |
| Cellular Localization | Nucleus, nucleoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SFRS2 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin embedded human lung cancer tissue slide using SFRS2 antibody at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS2 antibody at dilution of 1:3000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L), CL594-phalloidin (red).
IP
IP result of anti-SFRS2 (IP:4ug; Detection:1:300) with mouse brain tissue lysate 4000ug.| Application Notes | WB:1:1000-1:8000 IHC:1:50-1:500 ICC:1:1500-1:6000 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Buffer | Store at -20°C. Stable for one year after shipment. |
