WB
Variouslysates were subjected to SDS PAGE followed by western blotwith GAD1 antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblottedwith HRP-conjugated GAPDH Monoclonal antibody asloading control.IHC
Immunohistochemical analysis of paraffinembedded mouse cerebellum tissue slide using GAD1 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).IF-P
Immunofluorescent analysis of(4%PFA) fixed rat brain tissue using GAD1 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti Mouse IgG(H+L).| Product Name | GAD1 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | GAD1 fusion protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAIF-PIHCWB |
| WB:1:5000-1:50000 IHC:1:500-1:2000 IF-P:1:200-1:800 |
|
| Species Reactivity | HumanMousePigRabbitRat |
| Purification | Protein A |
| Gene Symbol | GAD1 |
|---|---|
| Gene Synonyms | GAD SCP CPSQ1 DEE89 |
| Gene Full Name | glutamate decarboxylase 1 |
| Gene Summary | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 67 kDa(Observed Molecular Weight:67 kDa, 57 kDa) |
WB
Variouslysates were subjected to SDS PAGE followed by western blotwith GAD1 antibody at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblottedwith HRP-conjugated GAPDH Monoclonal antibody asloading control.
IHC
Immunohistochemical analysis of paraffinembedded mouse cerebellum tissue slide using GAD1 antibody at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH 9.0).
IF-P
Immunofluorescent analysis of(4%PFA) fixed rat brain tissue using GAD1 antibody at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti Mouse IgG(H+L).| Application Notes | WB:1:5000-1:50000 IHC:1:500-1:2000 IF-P:1:200-1:800 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
