WB
Western blot analysis of Slow Skeletal Myosin Heavy chain on different lysates with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody at 1/1,000 dilution. Lane 1: Rat skeletal muscle tissue lysate Lane 2: Rat spleen tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 223 kDa Observed band size: 223 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody ) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Immunofluorescence analysis of frozen mouse skeletal muscle tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody ( green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Product Name | Slow Skeletal Myosin Heavy chain Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human MYH7 aa 1,250-1,300. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000 IHC:1:2000 IF-P:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MYH7 |
|---|---|
| Gene Synonyms | CMH1 MPD1 SPMD SPMM CMD1S MYHCB CMYO7A CMYO7B CMYP7A CMYP7B |
| Gene Full Name | myosin heavy chain 7 |
| Gene Summary | Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing distal myopathy. [provided by RefSeq, May 2022] |
| Alternative Names | Beta myosin heavy chain antibody β MHC antibody β-MHC antibody cardiac muscle beta isoform antibody CMD1S antibody CMH1 antibody MPD1 antibody MYH7 antibody MYH7_HUMAN antibody Myhc slow antibody Beta myosin heavy chain antibody β MHC antibody β-MHC antibody cardiac muscle beta isoform antibody CMD1S antibody CMH1 antibody MPD1 antibody MYH7 antibody MYH7_HUMAN antibody Myhc slow antibody MyHC-beta antibody MyHC-slow antibody MYHCB antibody Myopathy distal 1 antibody Myosin heavy chain (AA 1-96) antibody Myosin heavy chain 7 antibody Myosin heavy chain antibody Myosin heavy chain slow isoform antibody Myosin heavy chain cardiac muscle beta isoform antibody Myosin heavy chain 7 cardiac muscle beta antibody Myosin heavy polypeptide 7 cardiac muscle beta antibody Myosin-7 antibody Rhabdomyosarcoma antigen MU RMS 40.7A antibody SPMD antibody SPMM antibody |
| Molecular Weight(MW) | 223kDa |
| Cellular Localization | Cytoplasm, myofibril, sarcomere. |
WB
Western blot analysis of Slow Skeletal Myosin Heavy chain on different lysates with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody at 1/1,000 dilution. Lane 1: Rat skeletal muscle tissue lysate Lane 2: Rat spleen tissue lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 223 kDa Observed band size: 223 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody ) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Immunofluorescence analysis of frozen mouse skeletal muscle tissue with Rabbit anti-Slow Skeletal Myosin Heavy chain antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody ( green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).| Application Notes | WB:1:1000 IHC:1:2000 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
