WB
Western blot analysis of various lysates using Myosin heavy chain Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse heart using Myosin heavy chain Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IF-P
Immunofluorescence analysis of paraffin embedded mouse heart using Myosin heavy chain Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Myosin heavy chain Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1400 of human Myosin heavy chain (P12883). |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:1000-1:6000 IHC:1:100-1:500 IF-P:1:100-1:400 |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MYH7 |
|---|---|
| Gene Synonyms | CMH1 MPD1 SPMD SPMM CMD1S MYHCB CMYO7A CMYO7B CMYP7A CMYP7B |
| Gene Full Name | myosin heavy chain 7 |
| Gene Summary | Muscle myosin is a hexameric protein containing 2 heavy chain subunits, 2 alkali light chain subunits, and 2 regulatory light chain subunits. This gene encodes the beta (or slow) heavy chain subunit of cardiac myosin. It is expressed predominantly in normal human ventricle. It is also expressed in skeletal muscle tissues rich in slow-twitch type I muscle fibers. Changes in the relative abundance of this protein and the alpha (or fast) heavy subunit of cardiac myosin correlate with the contractile velocity of cardiac muscle. Its expression is also altered during thyroid hormone depletion and hemodynamic overloading. Mutations in this gene are associated with familial hypertrophic cardiomyopathy, myosin storage myopathy, dilated cardiomyopathy, and Laing distal myopathy. [provided by RefSeq, May 2022] |
| Alternative Names | CMD1S CMH1 MPD1 MYHCB SPMD SPMM Myosin heavy chain |
| Molecular Weight(MW) | 224kDa（Observed MW 250kDa） |
| Cellular Localization | Cytoplasm, myofibril. |
WB
Western blot analysis of various lysates using Myosin heavy chain Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse heart using Myosin heavy chain Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IF-P
Immunofluorescence analysis of paraffin embedded mouse heart using Myosin heavy chain Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:1000-1:6000 IHC:1:100-1:500 IF-P:1:100-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.02% Sodium azide, 50% glycerol, pH7.3. |
