WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIB3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)| Product Name | Anti-TRIB3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1-200 of human TRIB3(NP_066981) produced in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:2000 IHC:1:150 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein A/G |
| Gene Symbol | TRIB3 |
|---|---|
| Gene Synonyms | NIPK SINK TRB3 SKIP3 C20orf97 |
| Gene Full Name | tribbles pseudokinase 3 |
| Gene Summary | The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Alternative Names | C20orf97 NIPK SINK SKIP3 TRB 3 TRB3 TRIB3 Tribbles homolog 3 |
| Molecular Weight(MW) | 40kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIB3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)| Application Notes | WB:1:2000 IHC:1:150 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stable for 12 months from date of receipt. Store at -20°C as received. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
