WB
Western blot analysis using GLUL mouse mAb against Jurkat (1), and mouse liver (2) cell lysate.IHC
Immunohistochemical analysis of paraffin-embedded human cerebellar tissues using GLUL mouse mAb with DAB staining.ICC/IF
Immunofluorescence analysis of hela cells using GLUL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.FC
Flow cytometric analysis of Hela cells using GLUL mouse mAb (green) and negative control (red).| Product Name | Mouse Monoclonal Antibody to GLUL |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Purified recombinant fragment of human GLUL (AA: 2-121) expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:200-1:1000 ICC:1:200-1:1000 FC:1:200-1:400 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GLUL |
|---|---|
| Gene Synonyms | GS GLNS PIG43 PIG59 DEE116 |
| Gene Full Name | glutamate-ammonia ligase |
| Gene Summary | The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | GS GLNS PIG43 PIG59 |
| Molecular Weight(MW) | 42kDa |
| Cellular Localization | Cytoplasm,cytosol,Microsome,Mitochondrion,Cell membrane. |
WB
Western blot analysis using GLUL mouse mAb against Jurkat (1), and mouse liver (2) cell lysate.
IHC
Immunohistochemical analysis of paraffin-embedded human cerebellar tissues using GLUL mouse mAb with DAB staining.
ICC/IF
Immunofluorescence analysis of hela cells using GLUL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
FC
Flow cytometric analysis of Hela cells using GLUL mouse mAb (green) and negative control (red).| Application Notes | WB:1:500-1:2000 IHC:1:200-1:1000 ICC:1:200-1:1000 FC:1:200-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Storage Buffer | Purified antibody in PBS with 0.05% sodium azide. |
