WB
WB analysis of Jurkat using Glutamine Synthetase Monoclonal antibody WB result of Glutamine synthetase antibody (1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.WB
WB analysis of pig brain using Glutamine Synthetase Monoclonal antibody pig brain tissue were subjected to SDS PAGE followed by western blot with Glutamine Synthetase Monoclonal antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.IHC
IHC staining of human brain using Glutamine Synthetase Monoclonal antibody Immunohistochemical analysis of paraffin-embedded human brain tissue slide using Glutamine Synthetase Monoclonal antibody at dilution of 1:200 (under 10x lens).IF-F
IF Staining of zebrafish retina using Glutamine Synthetase Monoclonal antibody Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (magenta). Dilution 1:200. Glutamine synthetase (green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.IF-P
IF Staining of human brain using Glutamine Synthetase Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed human brain tissue using Glutamine Synthetase Monoclonal antibody at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).| Product Name | Glutamine Synthetase Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human Glutamine synthetase protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | IF-FIF-PIHCWB |
| WB:1:1000-1:8000 IHC:1:50-1:500 |
|
| Species Reactivity | HumanPigZebrafish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GLUL |
|---|---|
| Gene Synonyms | GS GLNS PIG43 PIG59 DEE116 |
| Gene Full Name | glutamate-ammonia ligase |
| Gene Summary | The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | GLNS GLUL Glutamate ammonia ligase Glutamate decarboxylase Glutamine synthetase GS PIG43 PIG59 |
| Molecular Weight(MW) | 374 aa, 42 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Endoplasmic reticulum,Membrane,Microsome,Mitochondrion. |
WB
WB analysis of Jurkat using Glutamine Synthetase Monoclonal antibody WB result of Glutamine synthetase antibody (1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.
WB
WB analysis of pig brain using Glutamine Synthetase Monoclonal antibody pig brain tissue were subjected to SDS PAGE followed by western blot with Glutamine Synthetase Monoclonal antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC
IHC staining of human brain using Glutamine Synthetase Monoclonal antibody Immunohistochemical analysis of paraffin-embedded human brain tissue slide using Glutamine Synthetase Monoclonal antibody at dilution of 1:200 (under 10x lens).
IF-F
IF Staining of zebrafish retina using Glutamine Synthetase Monoclonal antibody Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (magenta). Dilution 1:200. Glutamine synthetase (green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.
IF-P
IF Staining of human brain using Glutamine Synthetase Monoclonal antibody Immunofluorescent analysis of (4% PFA) fixed human brain tissue using Glutamine Synthetase Monoclonal antibody at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).| Application Notes | WB:1:1000-1:8000 IHC:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
