WB
Western blot analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Glutamine Synthetase/GLUL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody. Glutamine Synthetase/GLUL was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody. Glutamine Synthetase/GLUL was detected in a paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody. Glutamine Synthetase/GLUL was detected in a paraffin-embedded section of rat colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.ICC/IF
ICC/IF analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody . Glutamine Synthetase/GLUL was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of THP-1 cells using anti-Glutamine Synthetase/GLUL antibody . Overlay histogram showing THP-1 cells stained with Rabbit polyclonal antibody to Glutamine Synthetase/GLUL (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to Glutamine Synthetase/GLUL |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human GLUL recombinant protein (Position: N74-N373). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GLUL |
|---|---|
| Gene Synonyms | GS GLNS PIG43 PIG59 DEE116 |
| Gene Full Name | glutamate-ammonia ligase |
| Gene Summary | The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] |
| Alternative Names | GLNS GLUL Glutamate ammonia ligase Glutamate decarboxylase Glutamine synthetase GS PIG43 PIG59 |
| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Endoplasmic reticulum,Membrane,Microsom,Mitochondrion. |
WB
Western blot analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Glutamine Synthetase/GLUL antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody. Glutamine Synthetase/GLUL was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody. Glutamine Synthetase/GLUL was detected in a paraffin-embedded section of mouse colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody. Glutamine Synthetase/GLUL was detected in a paraffin-embedded section of rat colon tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ICC/IF
ICC/IF analysis of Glutamine Synthetase/GLUL using anti-Glutamine Synthetase/GLUL antibody . Glutamine Synthetase/GLUL was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of THP-1 cells using anti-Glutamine Synthetase/GLUL antibody . Overlay histogram showing THP-1 cells stained with Rabbit polyclonal antibody to Glutamine Synthetase/GLUL (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glutamine Synthetase/GLUL Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
