WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAD1 monoclonal antibody.IHC
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min.ICC/IF
Immunofluorescent staining of A549 cells using anti-GAD1 mouse monoclonal antibody.| Product Name | GAD67 (GAD1) Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Full length human recombinant protein of human GAD1 (NP_000808) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:50 ICC:1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GAD1 |
|---|---|
| Gene Synonyms | EP10 Gad-1 |
| Gene Full Name | glutamate decarboxylase 1 |
| Gene Summary | The protein encoded by this gene is an enzyme that catalyzes the production of gamma-aminobutyric acid from L-glutamic acid. Alternative splicing of this gene results in three products, the predominant 67 kDa isoform, a smaller 25 kDa isoform, and an even smaller 49 aa isoform. The two shorter isoforms are not thought to retain catalytic activity. [provided by RefSeq, Apr 2023] |
| Alternative Names | CPSQ1 DEE89 GAD SCP |
| Molecular Weight(MW) | 67kDa |
| Cellular Localization | cell cortex, clathrin-sculpted gamma-aminobutyric acid transport vesicle membrane, cytoplasm, GABA-ergic synapse, inhibitory synapse, perinuclear region of cytoplasm, plasma membrane, presynaptic active zone, vesicle membrane. |
WB
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GAD1 monoclonal antibody.
IHC
Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-GAD1 mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min.
ICC/IF
Immunofluorescent staining of A549 cells using anti-GAD1 mouse monoclonal antibody.| Application Notes | WB:1:1000-1:2000 IHC:1:50 ICC:1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C as received. Stable for 12 months from date of receipt. |
| Storage Buffer | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
