WB
Various lysates were subjected to SDS PAGE followed by western blot with SEPT6 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEPT6 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).| Product Name | SEPT6 Monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SEPT6 protein |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| WB:1:5000-1:50000 ICC/IF:1:400-1:1600 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | SEPTIN6 |
|---|---|
| Gene Synonyms | SEP2 SEPT2 SEPT6 Septin-6 |
| Gene Full Name | septin 6 |
| Gene Summary | This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 50kDa |
| Cellular Localization | Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SEPT6 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody as loading control.
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEPT6 antibody at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).| Application Notes | WB:1:5000-1:50000 ICC/IF:1:400-1:1600 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Data sheet for OM643847