WB
Western blot analysis of various lysates using IGF2BP2/IMP2 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.IHC
Immunohistochemistry analysis of paraffin embedded Mouse liver using IGF2BP2/IMP2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.ICC/IF
Immunofluorescence analysis of U2OS cells using IGF2BP2/IMP2 Rabbit pAb at dilution of 1:100. Secondary antibody: Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | IGF2BP2/IMP2 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:200-1:2000 IHC:1:50-1:100 ICC/IF:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IGF2BP2 |
|---|---|
| Gene Synonyms | IMP2 IMP-2 VICKZ2 |
| Gene Full Name | insulin like growth factor 2 mRNA binding protein 2 |
| Gene Summary | This gene encodes a protein that binds the 5' UTR of insulin-like growth factor 2 (IGF2) mRNA and regulates its translation. It plays an important role in metabolism and variation in this gene is associated with susceptibility to diabetes. Alternative splicing and promoter usage results in multiple transcript variants. Related pseudogenes are found on several chromosomes. [provided by RefSeq, Sep 2016] |
| Alternative Names | IMP2 IMP-2 VICKZ2 IGF2BP2/IMP2 |
| Molecular Weight(MW) | 66kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of various lysates using IGF2BP2/IMP2 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
IHC
Immunohistochemistry analysis of paraffin embedded Mouse liver using IGF2BP2/IMP2 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of U2OS cells using IGF2BP2/IMP2 Rabbit pAb at dilution of 1:100. Secondary antibody: Omnimabs® 488 Goat Anti-Rabbit IgG(H&L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:200-1:2000 IHC:1:50-1:100 ICC/IF:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
