WB
Western blot analysis of various lysates using IGF2BP2/IMP2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30 s.IHC
Immunohistochemistry analysis of paraffin embedded Human colon using IGF2BP2/IMP2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.IP
Immunoprecipitation of IGF2BP2/IMP2 from 300 µg extracts of Hep G2 cells was performed using 2 µg of IGF2BP2/IMP2 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using IGF2BP2/IMP2 Rabbit mAb at a dilution of 1:1000.| Product Name | IGF2BP2/IMP2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| WB:1:1000-1:4000 IHC:1:100-1:1000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IGF2BP2 |
|---|---|
| Gene Synonyms | IMP2 IMP-2 VICKZ2 |
| Gene Full Name | insulin like growth factor 2 mRNA binding protein 2 |
| Gene Summary | This gene encodes a protein that binds the 5' UTR of insulin-like growth factor 2 (IGF2) mRNA and regulates its translation. It plays an important role in metabolism and variation in this gene is associated with susceptibility to diabetes. Alternative splicing and promoter usage results in multiple transcript variants. Related pseudogenes are found on several chromosomes. [provided by RefSeq, Sep 2016] |
| Alternative Names | IMP2 IMP-2 VICKZ2 IGF2BP2/IMP2 |
| Molecular Weight(MW) | 60-75 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
WB
Western blot analysis of various lysates using IGF2BP2/IMP2 Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30 s.
IHC
Immunohistochemistry analysis of paraffin embedded Human colon using IGF2BP2/IMP2 Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
IP
Immunoprecipitation of IGF2BP2/IMP2 from 300 µg extracts of Hep G2 cells was performed using 2 µg of IGF2BP2/IMP2 Rabbit mAb. Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using IGF2BP2/IMP2 Rabbit mAb at a dilution of 1:1000.| Application Notes | WB:1:1000-1:4000 IHC:1:100-1:1000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
