WB
Western blot analysis of PDE4B on different lysates with Rabbit anti-PDE4B antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: Human brain tissue lysate (40 µg/Lane), Lane 3: Mouse brain tissue lysate (40 µg/Lane), Lane 4: Mouse heart tissue lysate (40 µg/Lane), Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDE4B antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.FC
Flow cytometric analysis of A549 cells labeling PDE4B. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | PDE4B Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fragment within Human PDE4B aa 550 to the C-terminus. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:1000 IHC:1:1000 FC:1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PDE4B |
|---|---|
| Gene Synonyms | DPDE4 PDEIVB |
| Gene Full Name | phosphodiesterase 4B |
| Gene Summary | This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014] |
| Alternative Names | 5'-cyclic phosphodiesterase 4B antibody cAMP-specific 3' antibody cAMP-specific 3' 5'-cyclic phosphodiesterase 4B antibody cAMP-specific phosphodiesterase-4 B isoform antibody DKFZp686F2182 antibody DPDE4 antibody dunce-like phosphodiesterase E4 antibody MGC126529 antibody OTTHUMP00000010652 antibody OTTHUMP00000010653 antibody 5'-cyclic phosphodiesterase 4B antibody cAMP-specific 3' antibody cAMP-specific 3' 5'-cyclic phosphodiesterase 4B antibody cAMP-specific phosphodiesterase-4 B isoform antibody DKFZp686F2182 antibody DPDE4 antibody dunce-like phosphodiesterase E4 antibody MGC126529 antibody OTTHUMP00000010652 antibody OTTHUMP00000010653 antibody OTTHUMP00000010654 antibody OTTHUMP00000010656 antibody OTTHUMP00000232365 antibody PDE 4B antibody PDE IVB antibody PDE32 antibody Pde4b antibody PDE4B_HUMAN antibody PDE4B5 antibody PDEIVB antibody Phosphodiesterase 4B antibody Phosphodiesterase 4B cAMP specific antibody Phosphodiesterase 4B cAMP specific (phosphodiesterase E4 dunce homolog Drosophila) antibody |
| Molecular Weight(MW) | 83 kDa |
| Cellular Localization | Cytoplasm, Cell membrane. |
WB
Western blot analysis of PDE4B on different lysates with Rabbit anti-PDE4B antibody at 1/1,000 dilution. Lane 1: A549 cell lysate (20 µg/Lane), Lane 2: Human brain tissue lysate (40 µg/Lane), Lane 3: Mouse brain tissue lysate (40 µg/Lane), Lane 4: Mouse heart tissue lysate (40 µg/Lane), Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PDE4B antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
FC
Flow cytometric analysis of A549 cells labeling PDE4B. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:1000 FC:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
