WB
Western Blot, Positive WB detected in: Hela whole cell lysate(30µg), U87 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), A549 whole cell lysate(30µg), 293T whole cell lysate(30µg), HepG2 whole cell lysate(30µg),Mouse Liver tissue lysate(30µg),Mouse Kidney tissue lysate(30µg). All lanes: SPPL3 antibody at 1:1000. Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution.Exposure time: 120sWB
Western Blot,Positive WB detected in: Mouse Liver tissue lysate(30µg),Mouse Kidney tissue lysate(30µg),Mouse Brain tissue lysate(30µg),Rat Liver tissue lysate(30µg),Rat Kidney tissue lysate(30µg),Rat Brain tissue lysate(30µg). All lanes: SPPL3 antibody at 1:1000. Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution.Exposure time: 3s| Product Name | Rabbit anti-SPPL3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Signal peptide peptidase-like 3 protein (213-263AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPPL3 |
|---|---|
| Gene Synonyms | IMP2 PSH1 PSL4 PRO4332 MDHV1887 |
| Gene Full Name | signal peptide peptidase like 3 |
| Gene Summary | Enables aspartic endopeptidase activity, intramembrane cleaving and protein homodimerization activity. Involved in T cell receptor signaling pathway; membrane protein proteolysis; and positive regulation of calcineurin-NFAT signaling cascade. Located in Golgi-associated vesicle membrane; endoplasmic reticulum; and plasma membrane. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | SPPL3 antibody IMP2 antibody PSL4 antibody Signal peptide peptidase-like 3 antibody SPP-like 3 antibody EC 3.4.23.- antibody Intramembrane protease 2 antibody IMP-2 antibody Presenilin homologous protein 1 antibody PSH1 antibody Presenilin-like protein 4 antibody |
| Molecular Weight(MW) | 57 kDa,19 kDa(Observed band size: 43 kDa) |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus. Membrane; Multi-pass membrane protein; Lumenal side. |
WB
Western Blot, Positive WB detected in: Hela whole cell lysate(30µg), U87 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), A549 whole cell lysate(30µg), 293T whole cell lysate(30µg), HepG2 whole cell lysate(30µg),Mouse Liver tissue lysate(30µg),Mouse Kidney tissue lysate(30µg). All lanes: SPPL3 antibody at 1:1000. Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution.Exposure time: 120s
WB
Western Blot,Positive WB detected in: Mouse Liver tissue lysate(30µg),Mouse Kidney tissue lysate(30µg),Mouse Brain tissue lysate(30µg),Rat Liver tissue lysate(30µg),Rat Kidney tissue lysate(30µg),Rat Brain tissue lysate(30µg). All lanes: SPPL3 antibody at 1:1000. Secondary, Goat polyclonal to rabbit IgG at 1/40000 dilution.Exposure time: 3s| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
