WB
Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb at 1:400 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.WB
Western blot analysis of lysates from C6 cells using Phospho-Histone H2AX-S139 Rabbit pAb at 1:400 dilution. C6 cells were treated with UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.IF
Immunofluorescence analysisof 293T(treated with UV) and293T(untreated) cells using Phospho-HistoneH2AX-S139 Rabbit pAb at a dilutionof 1:100 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.IHC
Immunohistochemistry analysis of paraffinembedded Human liver tissueusing Phospho-Histone H2AX-S139 RabbitpAb at a dilution of 1:100 (40xlens). High pressure antigen retrieval wasperformed with 0.01 M citrate buffer (pH 6.0)prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Rat lung tissue using PhosphoHistone H2AX-S139 Rabbit pAb at adilution of 1:100 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffinembedded Mouse heart tissueusing Phospho-Histone H2AX-S139 RabbitpAb at a dilution of 1:100 (40xlens). High pressure antigen retrieval wasperformed with 0.01 M citrate buffer (pH 6.0)prior to IHC staining.| Product Name | Phospho-Histone H2AX-S139 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | P-S139 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AX |
|---|---|
| Gene Synonyms | H2A.X H2A/X H2AFX |
| Gene Full Name | H2A.X variant histone |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015] |
| Molecular Weight(MW) | 15kDa(Observed band size:17kDa) |
| Cellular Localization | Chromosome, Nucleus. |
WB
Western blot analysis of lysates from NIH/3T3 cells using Phospho-Histone H2AX-S139 Rabbit pAb at 1:400 dilution. NIH/3T3 cells were treated with UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.
WB
Western blot analysis of lysates from C6 cells using Phospho-Histone H2AX-S139 Rabbit pAb at 1:400 dilution. C6 cells were treated with UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 20s.
IF
Immunofluorescence analysisof 293T(treated with UV) and293T(untreated) cells using Phospho-HistoneH2AX-S139 Rabbit pAb at a dilutionof 1:100 (40x lens). Secondary antibody:Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI fornuclear staining.
IHC
Immunohistochemistry analysis of paraffinembedded Human liver tissueusing Phospho-Histone H2AX-S139 RabbitpAb at a dilution of 1:100 (40xlens). High pressure antigen retrieval wasperformed with 0.01 M citrate buffer (pH 6.0)prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Rat lung tissue using PhosphoHistone H2AX-S139 Rabbit pAb at adilution of 1:100 (40x lens). High pressureantigen retrieval was performed with 0.01 Mcitrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffinembedded Mouse heart tissueusing Phospho-Histone H2AX-S139 RabbitpAb at a dilution of 1:100 (40xlens). High pressure antigen retrieval wasperformed with 0.01 M citrate buffer (pH 6.0)prior to IHC staining.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
